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Effect of zileuton and montelukast on <t>apoptosis.</t> At 24 h after SCI, spinal cord tissues obtained from SCI+vehicle-treated mice demonstrated a marked appearance of positive stain for annexin V FITC (a), an index of cells that are induced to undergo apoptosis. Some cells showed a positive intracellular staining to PI (a1), an index of cells in the late stage of apoptosis. On the contrary, spinal cord tissues section from mice treated with zileuton or montelukast demonstrate no cells in the earlier stage (b and c, respectively) of apoptosis and fewer cells (positive to PI) in the later stages of apoptosis (b1 and c1, respectively). (a2, b2, and c2) Staining combination of a and a1, b and b1, and c and c1, respectively. The figure is representative of at least three experiments performed on different experimental days. FITC, fluorescein isothiocyanate; PI, propidium iodide SCI, spinal cord injury.
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Effect of zileuton and montelukast on <t>apoptosis.</t> At 24 h after SCI, spinal cord tissues obtained from SCI+vehicle-treated mice demonstrated a marked appearance of positive stain for annexin V FITC (a), an index of cells that are induced to undergo apoptosis. Some cells showed a positive intracellular staining to PI (a1), an index of cells in the late stage of apoptosis. On the contrary, spinal cord tissues section from mice treated with zileuton or montelukast demonstrate no cells in the earlier stage (b and c, respectively) of apoptosis and fewer cells (positive to PI) in the later stages of apoptosis (b1 and c1, respectively). (a2, b2, and c2) Staining combination of a and a1, b and b1, and c and c1, respectively. The figure is representative of at least three experiments performed on different experimental days. FITC, fluorescein isothiocyanate; PI, propidium iodide SCI, spinal cord injury.
Nquery Version 8.5, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of zileuton and montelukast on <t>apoptosis.</t> At 24 h after SCI, spinal cord tissues obtained from SCI+vehicle-treated mice demonstrated a marked appearance of positive stain for annexin V FITC (a), an index of cells that are induced to undergo apoptosis. Some cells showed a positive intracellular staining to PI (a1), an index of cells in the late stage of apoptosis. On the contrary, spinal cord tissues section from mice treated with zileuton or montelukast demonstrate no cells in the earlier stage (b and c, respectively) of apoptosis and fewer cells (positive to PI) in the later stages of apoptosis (b1 and c1, respectively). (a2, b2, and c2) Staining combination of a and a1, b and b1, and c and c1, respectively. The figure is representative of at least three experiments performed on different experimental days. FITC, fluorescein isothiocyanate; PI, propidium iodide SCI, spinal cord injury.
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Effect of zileuton and montelukast on <t>apoptosis.</t> At 24 h after SCI, spinal cord tissues obtained from SCI+vehicle-treated mice demonstrated a marked appearance of positive stain for annexin V FITC (a), an index of cells that are induced to undergo apoptosis. Some cells showed a positive intracellular staining to PI (a1), an index of cells in the late stage of apoptosis. On the contrary, spinal cord tissues section from mice treated with zileuton or montelukast demonstrate no cells in the earlier stage (b and c, respectively) of apoptosis and fewer cells (positive to PI) in the later stages of apoptosis (b1 and c1, respectively). (a2, b2, and c2) Staining combination of a and a1, b and b1, and c and c1, respectively. The figure is representative of at least three experiments performed on different experimental days. FITC, fluorescein isothiocyanate; PI, propidium iodide SCI, spinal cord injury.
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GraphPad Software Inc nquery v 6
Effect of zileuton and montelukast on <t>apoptosis.</t> At 24 h after SCI, spinal cord tissues obtained from SCI+vehicle-treated mice demonstrated a marked appearance of positive stain for annexin V FITC (a), an index of cells that are induced to undergo apoptosis. Some cells showed a positive intracellular staining to PI (a1), an index of cells in the late stage of apoptosis. On the contrary, spinal cord tissues section from mice treated with zileuton or montelukast demonstrate no cells in the earlier stage (b and c, respectively) of apoptosis and fewer cells (positive to PI) in the later stages of apoptosis (b1 and c1, respectively). (a2, b2, and c2) Staining combination of a and a1, b and b1, and c and c1, respectively. The figure is representative of at least three experiments performed on different experimental days. FITC, fluorescein isothiocyanate; PI, propidium iodide SCI, spinal cord injury.
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GraphPad Software Inc nquery advisor 7.0
Effect of zileuton and montelukast on <t>apoptosis.</t> At 24 h after SCI, spinal cord tissues obtained from SCI+vehicle-treated mice demonstrated a marked appearance of positive stain for annexin V FITC (a), an index of cells that are induced to undergo apoptosis. Some cells showed a positive intracellular staining to PI (a1), an index of cells in the late stage of apoptosis. On the contrary, spinal cord tissues section from mice treated with zileuton or montelukast demonstrate no cells in the earlier stage (b and c, respectively) of apoptosis and fewer cells (positive to PI) in the later stages of apoptosis (b1 and c1, respectively). (a2, b2, and c2) Staining combination of a and a1, b and b1, and c and c1, respectively. The figure is representative of at least three experiments performed on different experimental days. FITC, fluorescein isothiocyanate; PI, propidium iodide SCI, spinal cord injury.
Nquery Advisor 7.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Rio Tinto laterrière works
Effect of zileuton and montelukast on <t>apoptosis.</t> At 24 h after SCI, spinal cord tissues obtained from SCI+vehicle-treated mice demonstrated a marked appearance of positive stain for annexin V FITC (a), an index of cells that are induced to undergo apoptosis. Some cells showed a positive intracellular staining to PI (a1), an index of cells in the late stage of apoptosis. On the contrary, spinal cord tissues section from mice treated with zileuton or montelukast demonstrate no cells in the earlier stage (b and c, respectively) of apoptosis and fewer cells (positive to PI) in the later stages of apoptosis (b1 and c1, respectively). (a2, b2, and c2) Staining combination of a and a1, b and b1, and c and c1, respectively. The figure is representative of at least three experiments performed on different experimental days. FITC, fluorescein isothiocyanate; PI, propidium iodide SCI, spinal cord injury.
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Image Search Results


Effect of zileuton and montelukast on apoptosis. At 24 h after SCI, spinal cord tissues obtained from SCI+vehicle-treated mice demonstrated a marked appearance of positive stain for annexin V FITC (a), an index of cells that are induced to undergo apoptosis. Some cells showed a positive intracellular staining to PI (a1), an index of cells in the late stage of apoptosis. On the contrary, spinal cord tissues section from mice treated with zileuton or montelukast demonstrate no cells in the earlier stage (b and c, respectively) of apoptosis and fewer cells (positive to PI) in the later stages of apoptosis (b1 and c1, respectively). (a2, b2, and c2) Staining combination of a and a1, b and b1, and c and c1, respectively. The figure is representative of at least three experiments performed on different experimental days. FITC, fluorescein isothiocyanate; PI, propidium iodide SCI, spinal cord injury.

Journal:

Article Title: Effects of zileuton and montelukast in mouse experimental spinal cord injury

doi: 10.1038/sj.bjp.0707577

Figure Lengend Snippet: Effect of zileuton and montelukast on apoptosis. At 24 h after SCI, spinal cord tissues obtained from SCI+vehicle-treated mice demonstrated a marked appearance of positive stain for annexin V FITC (a), an index of cells that are induced to undergo apoptosis. Some cells showed a positive intracellular staining to PI (a1), an index of cells in the late stage of apoptosis. On the contrary, spinal cord tissues section from mice treated with zileuton or montelukast demonstrate no cells in the earlier stage (b and c, respectively) of apoptosis and fewer cells (positive to PI) in the later stages of apoptosis (b1 and c1, respectively). (a2, b2, and c2) Staining combination of a and a1, b and b1, and c and c1, respectively. The figure is representative of at least three experiments performed on different experimental days. FITC, fluorescein isothiocyanate; PI, propidium iodide SCI, spinal cord injury.

Article Snippet: The binding of annexin-V fluorescein isothiocyanate (FITC) (Ann-V) to externalized phosphatidylserine was used as a measurement of apoptosis in spinal cord tissue section with an Ann-V-propidium iodide (PI) apoptosis detection kit (Santa Cruz, DBA, Milan, Italy) according to the manufacturer's instructions.

Techniques: Staining